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Semester | spring semester 2020 |
Course frequency | Every spring sem. |
Lecturers |
Timothy Sharpe (timothy.sharpe@unibas.ch, Assessor)
Ludovit Zweifel (ludovit.zweifel@unibas.ch) |
Content | This four-day course provides a practical and theoretical introduction of a range of biophysical methods that can be used to measure the stability, size, interactions and dynamics of biomolecules. Students will gain hands-on experience of different techniques, an idea of how those techniques can complement each other, and opportunities to discuss which techniques might be suitable for their own projects. The techniques covered in the course will be: isothermal titration calorimetry (ITC), microscale thermophoresis (MST), surface plasmon resonance (SPR), differential scanning fluorimetry (DSF), analytical ultracentrifugation (AUC), dynamic light scattering (DLS), multi-angle light-scattering (MALS), fluorescence correlation spectroscopy (FCS), and fluorescence lifetime imaging (FLIM). At the end of the course, students will be required to write a short proposal (2-4 pages) for the application of at least one of the techniques covered in the course to their own research project. The proposal should demonstrate a theoretical understanding of the technique, consideration of the sample requirements and experimental protocol, and ideas about the potential meaning of the results within the context of their project. Day 1: Theory of binding interactions; ITC and MST experiments. Day 2: Other methods to study binding; Advanced data analysis; Theory of protein stability; DSF experiments. Day 3: Theory of mass and size determination; AUC data analysis; DLS and MALS experiments. Day 4: Theory of time-resolved fluorescence imaging; FCS and FLIM experiments. |
Weblink | Graduate Teaching Program |
Admission requirements | The courses are limited to a maximum of 4 participants, PhD students only. The course must be fully attended, otherwise the CPs can not be awarded. |
Course application | Please register via: Angelika Klarer (angie.klarer@unibas.ch) Klingelbergstrasse 70 4056 Basel |
Language of instruction | English |
Use of digital media | No specific media used |
Interval | Weekday | Time | Room |
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No dates available. Please contact the lecturer.
Modules |
Cycle I: Practical and Experimental Skills (Master's Studies: Molecular Biology) Doctorate Biochemistry: Recommendations (PhD subject: Biochemistry) Doctorate Biophysics: Recommendations (PhD subject: Biophysics) Doctorate Cell Biology: Recommendations (PhD subject: Cell Biology) Doctorate Computational Biology: Recommendations (PhD subject: Computational Biology) Doctorate Genetics: Recommendations (PhD subject: Genetics) Doctorate Microbiology: Recommendations (PhD subject: Microbiology) Doctorate Molecular Biology: Recommendations (PhD subject: Molecular Biology) Doctorate Neurobiology: Recommendations (PhD subject: Neurobiology) Doctorate Pharmacology: Recommendations (PhD subject: Pharmacology) Doctorate Structural Biology: Recommendations (PhD subject: Structural Biology) |
Assessment format | continuous assessment |
Assessment registration/deregistration | Reg.: course registration, dereg: cancel course registration |
Repeat examination | no repeat examination |
Scale | Pass / Fail |
Repeated registration | as often as necessary |
Responsible faculty | Faculty of Science, studiendekanat-philnat@unibas.ch |
Offered by | Departement Biozentrum |