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38238-01 - Praktikum: Basics in recombinant protein production (3 KP)
| Semester | Herbstsemester 2016 |
| Angebotsmuster | unregelmässig |
| Dozierende |
Marc Creus (marc.creus@unibas.ch)
Daniel Häussinger (daniel.haeussinger@unibas.ch) Florian Peter Seebeck (florian.seebeck@unibas.ch, BeurteilerIn) |
| Inhalt | Bio-based technologies will play a central role in future industries. Microorganisms have diversified for 3.5 billion years and evolved molecular systems to utilize different energy forms, to build complex molecular structure from diverse feedstocks and to recycle any type of biomass to basic minerals. We can exploit this molecular diversity as a toolbox for sustainable and innovative bio-based production of energy, materials, fine chemicals, food, or pharmaceuticals. In this context, recombinant protein production is a key technology because it allows the researcher access virtually any protein encoded by over 10000 sequenced microbial genomes. This course should train interested beginners in recombinant protein production techniques. |
| Lernziele | This course is an first introduction to production and characterization of recombinant proteins. This course should enable students to search specific protein sequences by structure based genome mining, visualize protein structures, produce the corresponding protein in the lab, and analyze its homogeneity, stability and activity by a series of basic techniques. |
| Literatur | Clark & Pazdernik: Molekulare Biotechnologie, Spektrum Verlag Click, Pasternak & Patten: Molecular Biotechnology, ASM press Lesk: Bioinformatik, Spektrum Verlag Sezonov, Joseleau-Petit, Dari, J. Bacteriol. 2007, 189 (23) 8746 – 8749 Studier, Protein Expr Purif. 2005, 41(1) 207 – 34 Carlson et al.: J Biol Chem. 2008, 283 (29) 20117 – 20125 Pace, Vajdos, Fee, Grimsley & Gray: Protein Sci. 1995, 4 (11), 2411 – 2423 Riener, Kada & Gruber: Anal. Bioanal. Chem. 2002, 373, 266 – 76 |
| Bemerkungen | Der Kurs ist auf sechs Teilnehmer beschränkt. |
| Teilnahmevoraussetzungen | Bachelor degree in Chemistry/Nanosciences and related areas. |
| Anmeldung zur Lehrveranstaltung | Please indicate you interest by email (florian.seebeck@unibas.ch) this will help us to plan the lab corse. Also you need to enroll through MOnA. |
| Unterrichtssprache | Englisch |
| Einsatz digitaler Medien | kein spezifischer Einsatz |
| Intervall | Wochentag | Zeit | Raum |
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Keine Einzeltermine verfügbar, bitte informieren Sie sich direkt bei den Dozierenden.
| Module |
Modul Vertiefungsfach Chemie (Master Nanowissenschaften) Vertiefungsmodul (Master Chemie) |
| Prüfung | Lehrveranst.-begleitend |
| Hinweise zur Prüfung | The students will present their results in a group seminar at the end of the course and are requested to submit a written report. |
| An-/Abmeldung zur Prüfung | Anm.: Belegen Lehrveranstaltung; Abm.: stornieren |
| Wiederholungsprüfung | keine Wiederholungsprüfung |
| Skala | Pass / Fail |
| Belegen bei Nichtbestehen | beliebig wiederholbar |
| Zuständige Fakultät | Philosophisch-Naturwissenschaftliche Fakultät, studiendekanat-philnat@unibas.ch |
| Anbietende Organisationseinheit | Departement Chemie |