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Semester | Frühjahrsemester 2022 |
Angebotsmuster | Jedes Frühjahrsem. |
Dozierende |
Timothy Sharpe (timothy.sharpe@unibas.ch, BeurteilerIn)
Ludovit Zweifel (ludovit.zweifel@unibas.ch) |
Inhalt | This course provides a practical and theoretical introduction of a range of biophysical methods that can be used to measure the interactions, stability and size of biomolecules. Students will gain hands-on experience of different techniques, an idea of how those techniques can complement each other, and opportunities to discuss which techniques might be suitable for their own projects. The techniques covered in the course will be: isothermal titration calorimetry (ITC), microscale thermophoresis (MST), surface plasmon resonance (SPR), differential scanning fluorimetry (DSF), dynamic light scattering (DLS), multi-angle light-scattering (MALS), analytical ultracentrifugation (AUC), and mass photometry. At the end of the course, students will be required to write a short proposal (2-4 pages) for the application of at least one of the techniques covered in the course to their own research project. The proposal should demonstrate a theoretical understanding of the technique, consideration of the sample requirements and experimental protocol, and ideas about the potential meaning of the results within the context of their project. Day 1: Overview of methods for biomolecule interactions; Overview of BF resources for users; ITC theory; ITC experiment. Day 2: MST theory; MST experiment; Overview of other methods for studying binding; discussion of more advanced data analysis (global fitting, error analysis). Day 3: Overview of stability screening; DSF theory; DSF experiment; DLS theory; DLS experiment Day 4: Overview of mass and size determination; MALS theory; MALS experiment; Mass Photometry theory and experiment; AUC theory and data analysis. |
Bemerkungen | Please register for the course via phd-biozentrum@unibas.ch and indicate your preferred course dates. Course 1: 04.04.-07.04.2022 Course 2: 25.04.-28.04.2022 Both courses are fully booked! |
Weblink | Graduate Teaching Program |
Teilnahmebedingungen | The courses are limited to a maximum of 4 participants, PhD students only. The course must be fully attended, otherwise the CPs can not be awarded. |
Unterrichtssprache | Englisch |
Einsatz digitaler Medien | kein spezifischer Einsatz |
Intervall | Wochentag | Zeit | Raum |
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Bemerkungen | Course dates: Course 1: 04.04.-07.04.2022 Course 2: 25.04.-28.04.2022 Both courses are fully booked! |
Keine Einzeltermine verfügbar, bitte informieren Sie sich direkt bei den Dozierenden.
Module |
Cycle I: Practical and Experimental Skills (Masterstudium: Molekularbiologie) Doktorat Biochemie: Empfehlungen (Promotionsfach: Biochemie) Doktorat Biophysik: Empfehlungen (Promotionsfach: Biophysik) Doktorat Computational Biology: Empfehlungen (Promotionsfach: Computational Biology) Doktorat Genetik: Empfehlungen (Promotionsfach: Genetik) Doktorat Mikrobiologie: Empfehlungen (Promotionsfach: Mikrobiologie) Doktorat Molekularbiologie: Empfehlungen (Promotionsfach: Molekularbiologie) Doktorat Neurobiologie: Empfehlungen (Promotionsfach: Neurobiologie) Doktorat Pharmakologie: Empfehlungen (Promotionsfach: Pharmakologie) Doktorat Strukturbiologie: Empfehlungen (Promotionsfach: Strukturbiologie) Doktorat Zellbiologie: Empfehlungen (Promotionsfach: Zellbiologie) |
Leistungsüberprüfung | Lehrveranst.-begleitend |
An-/Abmeldung zur Leistungsüberprüfung | Anm.: Belegen Lehrveranstaltung; Abm.: stornieren |
Wiederholungsprüfung | keine Wiederholungsprüfung |
Skala | Pass / Fail |
Wiederholtes Belegen | beliebig wiederholbar |
Zuständige Fakultät | Philosophisch-Naturwissenschaftliche Fakultät, studiendekanat-philnat@unibas.ch |
Anbietende Organisationseinheit | Departement Biozentrum |