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68795-02 - Praktikum: Experimental Molecular Biology (12 KP)
| Semester | Frühjahrsemester 2024 |
| Angebotsmuster | Jahreskurs, beginnt im Herbstsemester |
| Dozierende |
Dominik Buser (dominik-pascal.buser@unibas.ch, BeurteilerIn)
Alexander Schier (alex.schier@unibas.ch) |
| Inhalt | • Repetition of acquired skills in "Experimental Molecular Biology (68795-01)" • Bacterial protein expression: IPTG induction and IMAC • Molecular cloning: DNA plasmid isolation/purification and Sanger sequencing • Mammalian cell culture: Culturing; Splitting; Counting and Seeding cells; Plasmid transfection; Protein expression; Cell lysis • Protein analysis: Protein concentration determination; Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE); Western Blotting; Enhanced chemiluminescence (ECL); Coomassie staining; Silver staining • Protein pulldown analysis • AP-MS/Proteomics: Theory and practice of mass spectrometry and proteomics; Pulldown with streptavidin beads; Reduction and tryptic digestion; Peptide purification and desalting; Preparation of samples for MS loading • Preparation and presentation of collected data • Reading, presenting, and understanding scientific articles • Experimental design • Troubleshooting |
| Lernziele | Aim of the "Experimental Molecular Biology (EMB)" course is to train Biology students for the gradual transition from a student to a scientist, including training technical as well as scientific/intellectual skills. By combining these skills, students will dissect the function, localization, and interaction network of a protein of interest. Technical skills include essential lab skills, starting with pipetting, making buffers, cloning, protein expression in mammalian cell culture, standard biochemical techniques, as well as more advanced techniques such as microscopy and proteomics. Scientific/intellectual skills comprise sharpening student’s mindset in planning and interpreting experiments, as well as reading, presenting, and discussing literature and scientific articles. |
| Literatur | Lectures, protocols, other information, and the agenda will be provided using an internal platform and on ADAM (https://adam.unibas.ch). |
| Bemerkungen | Pregnant and nursing mothers should inform the assessor of the course at least two weeks prior to EMB course start. Students must have an accident insurance. Students requiring adaptive measures due to a handicap or chronic disorder should inform the assessor after registration. The “StoB (Studieren ohne Barrieren)” service center is available for your needs and assistance. In case of sickness, inform the assessor by mail with a letter of absence including a sick note (attest). For introduction into proteomics, we will host Dr. Alex Schmidt and the Proteomics Core Facility. In addition, for a literature search seminar, we will host PD Dr. Christian Appenzeller-Herzog and Dr. Nuria Plattner. |
| Teilnahmevoraussetzungen | This course can be only attended if the "Experimental Molecular Biology (68795-01)" was completed (during fall semesters). |
| Anmeldung zur Lehrveranstaltung | EMB course (68795-02) can be only attended if the former course (68795-01) has been completed. |
| Unterrichtssprache | Englisch |
| Einsatz digitaler Medien | kein spezifischer Einsatz |
| Intervall | Wochentag | Zeit | Raum |
|---|---|---|---|
| wöchentlich | Dienstag | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| wöchentlich | Mittwoch | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Bemerkungen | Two afternoons per week (Tuesday and Wednesday). Rooms and additional information will be announced on our internal course website. |
Einzeltermine
| Datum | Zeit | Raum |
|---|---|---|
| Dienstag 27.02.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Mittwoch 28.02.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Dienstag 05.03.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Mittwoch 06.03.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Dienstag 12.03.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Mittwoch 13.03.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Dienstag 19.03.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Mittwoch 20.03.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Dienstag 26.03.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Mittwoch 27.03.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Dienstag 02.04.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Mittwoch 03.04.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Dienstag 09.04.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Mittwoch 10.04.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Dienstag 16.04.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Mittwoch 17.04.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Dienstag 23.04.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Mittwoch 24.04.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Dienstag 30.04.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Mittwoch 01.05.2024 | 13.15-18.00 Uhr | Tag der Arbeit |
| Mittwoch 01.05.2024 | 13.15-18.00 Uhr | Tag der Arbeit |
| Dienstag 07.05.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Mittwoch 08.05.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Dienstag 14.05.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Mittwoch 15.05.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Dienstag 21.05.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Mittwoch 22.05.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Dienstag 28.05.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Mittwoch 29.05.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Module |
Modul: Computational Biology (Bachelorstudium: Computational Sciences) Modul: Molekularbiologie (Bachelorstudium: Biologie) |
| Prüfung | Lehrveranst.-begleitend |
| Hinweise zur Prüfung | To pass "Experimental Molecular Biology (68795-02)", the following points are required: • Attendance of class • Writing and documenting experiments in a lab journal on a weekly basis • Active participation in the lab and in class • Preparation and presentation of literature (Journal Club) and research seminars • Leading good laboratory practice "Experimental Molecular Biology (68795-02)" can be only attended after completion of "Experimental Molecular Biology (68795-01)". |
| An-/Abmeldung zur Prüfung | Anm.: Belegen Lehrveranstaltung; Abm.: stornieren |
| Wiederholungsprüfung | keine Wiederholungsprüfung |
| Skala | Pass / Fail |
| Belegen bei Nichtbestehen | beliebig wiederholbar |
| Zuständige Fakultät | Philosophisch-Naturwissenschaftliche Fakultät, studiendekanat-philnat@unibas.ch |
| Anbietende Organisationseinheit | Departement Biozentrum |