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72507-01 - Vorlesung: (Bio-)Molecular Behaviour at the Nanoscale 3 KP

Semester Herbstsemester 2024
Angebotsmuster unregelmässig
Dozierende Sonja Schmid (sonja.schmid@unibas.ch, BeurteilerIn)
Yang Yao (yang.yao@unibas.ch)
Inhalt This MSc lecture provides an interdisciplinary overview of the behaviour of molecules and biomolecules at the nanoscale and the latest techniques to study those.

In the 1st part of the course, we will discuss the crystallization and molecular dynamics of polymers and water in both hard nanoconfinement and soft nanoconfinement. Through the curriculum, the students will learn the techniques used to study these phenomena, including broadband dielectric spectroscopy, infrared spectroscopy, differential scanning calorimetry, and X-ray scattering.

The 2nd part of the course zooms in on bio-macromolecules (proteins, DNA, RNA), their functional dynamics in a ‘jiggling and wiggling’ cellular environment, and also the techniques to reveal and study those with single-molecule resolution. The students will learn the working principles and concepts behind various frontier single-molecule techniques that have revolutionized the way we can monitor inter- and intra-molecular dynamics in the life-sciences:
• single-molecule fluorescence imaging and FRET spectroscopies
• nanopore technology, incl. biological and solid-state pores
• high-speed atomic force microscopy (AFM) imaging
• force spectroscopies, optical and magnetic tweezers, AFM, AFS, etc.
In each case, the required background will be introduced followed by an emphasis on information gain and applications.
Lernziele • Gaining intuition about the key differences between the behaviour of macroscopic objects known from daily life and the behaviour of (bio-)molecules in nanoscale environments, such as the living cell.
• Understand the fundamental principles governing the crystallization and molecular dynamics of polymers and water at the nanoscale, focusing on how confinement influences these properties.
• Gain knowledge on broadband dielectric spectroscopy and its application in investigating the molecular dynamics of molecules in various (biologically relevant) environmental conditions.
• Acquire knowledge of differential scanning calorimetry to assess the thermal properties and transitions in nanoscale systems.
• Gaining an overview about bio-nano techniques with single-molecule resolution.
• Being able to explain the unique benefits of single-molecule resolution and the risks of ensemble averaging.
• Being able to design single-molecule experiments to address current research questions in the life sciences.
Literatur The course material will be provided on ADAM.
Relevant textbooks include:
• Biophysics in the cell. Bornschlögl, Dietz. 2024. Springer Berlin. ISBN-13: 978-3-662-65804-8
• Single-Molecule Techniques: A Laboratory Manual. Ha, Selvin. 2008. CSHL Press. ISBN 978-087969775-4
• Broadband dielectric spectroscopy. F. Kremer, A. Schönhals, 2003. Springer Berlin Heidelberg. ISBN 978-3-642-62809-2
• Dynamics in Geometrical Confinement. F. Kremer, 2014. Springer Cham. ISBN 978-3-319-06099-6
Bemerkungen Access to Rosental 1060, room 7.48 only via gate BPR 1047, Mattenstrasse 24a.
Students of the University of Basel have to register on services.unibas.ch for an access to the Rosental until 9 September 2024.

 

Teilnahmebedingungen BSc. level chemistry, molecular life sciences, physics courses
Anmeldung zur Lehrveranstaltung Register on services.unibas.ch
Registration until 9 September 2024 for access to the Rosental campus.
Unterrichtssprache Englisch
Einsatz digitaler Medien kein spezifischer Einsatz

 

Intervall Wochentag Zeit Raum
wöchentlich Freitag 10.15-13.00 Campus Rosental 1060, Seminarraum 7.48

Einzeltermine

Datum Zeit Raum
Freitag 20.09.2024 10.15-13.00 Uhr Campus Rosental 1060, Seminarraum 7.48
Freitag 27.09.2024 10.15-13.00 Uhr Campus Rosental 1060, Seminarraum 7.48
Freitag 04.10.2024 10.15-13.00 Uhr Campus Rosental 1060, Seminarraum 7.48
Freitag 11.10.2024 10.15-13.00 Uhr Campus Rosental 1060, Seminarraum 7.48
Freitag 18.10.2024 10.15-13.00 Uhr Campus Rosental 1060, Seminarraum 7.48
Freitag 25.10.2024 10.15-13.00 Uhr Campus Rosental 1060, Seminarraum 7.48
Freitag 01.11.2024 10.15-13.00 Uhr Campus Rosental 1060, Seminarraum 7.48
Freitag 08.11.2024 10.15-13.00 Uhr Campus Rosental 1060, Seminarraum 7.48
Freitag 15.11.2024 10.15-13.00 Uhr Campus Rosental 1060, Seminarraum 7.48
Freitag 22.11.2024 10.15-13.00 Uhr Campus Rosental 1060, Seminarraum 7.48
Freitag 29.11.2024 10.15-13.00 Uhr Dies Academicus
Freitag 06.12.2024 10.15-13.00 Uhr Campus Rosental 1060, Seminarraum 7.48
Freitag 13.12.2024 10.15-13.00 Uhr Campus Rosental 1060, Seminarraum 7.48
Freitag 20.12.2024 10.15-13.00 Uhr Campus Rosental 1060, Seminarraum 7.48
Module Doktorat Chemie: Empfehlungen (Promotionsfach: Chemie)
Modul: Physical Chemistry (Masterstudium: Chemie)
Leistungsüberprüfung Lehrveranst.-begleitend
Hinweise zur Leistungsüberprüfung The lectures are complemented with at least 1/3 interactive group work modules incl. a journal club and exercises during the lectures.
20%: interactive participation
80%: oral exam
An-/Abmeldung zur Leistungsüberprüfung Anm.: Belegen Lehrveranstaltung; Abm.: stornieren
Wiederholungsprüfung keine Wiederholungsprüfung
Skala 1-6 0,5
Wiederholtes Belegen beliebig wiederholbar
Zuständige Fakultät Philosophisch-Naturwissenschaftliche Fakultät, studiendekanat-philnat@unibas.ch
Anbietende Organisationseinheit Departement Chemie

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