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68795-01 - Praktikum: Experimental Molecular Biology (12 KP)
| Semester | Herbstsemester 2024 |
| Angebotsmuster | Jahreskurs, beginnt im Herbstsemester |
| Dozierende |
Oliver Biehlmaier (oliver.biehlmaier@unibas.ch)
Dominik Buser (dominik-pascal.buser@unibas.ch, BeurteilerIn) Alexia Loynton-Ferrand (alexia.ferrand@unibas.ch) Alexander Schier (alex.schier@unibas.ch) |
| Inhalt | • Media and buffer preparation for Molecular Biology • Molecular cloning: polymerase chain reaction (PCR), endonuclease/restriction enzyme digestion, agarose gel electrophoresis, ligation, bacterial transformation, DNA plasmid isolation/purification, Sanger sequencing • Mammalian cell culture: Culturing, splitting, counting and seeding cells, plasmid transfection, protein expression, cell lysis • Protein analysis: Protein concentration determination, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), Western Blotting, Enhanced chemiluminescence (ECL) • Light and Fluorescence Microscopy: Basics skills with light microscope, immunofluoresence (IF) staining • Scientific data illustration • Image analysis • Preparation and presentation of collected data • Reading and understanding literature • Experimental design • Troubleshooting |
| Lernziele | Aim of the Experimental Molecular Biology (EMB) course is to train Biology students for the gradual transition from a student to a scientist, including training technical as well as scientific/intellectual skills. By combining these skills, students will dissect the function, localization, and interaction network of a protein of interest. Technical skills include essential lab skills, starting with pipetting, making buffers, cloning, CRISPR/Cas9, protein expression in mammalian cell culture, standard biochemical techniques, as well as more advanced techniques such as microscopy and proteomics. Scientific/intellectual skills comprise sharpening student’s mindset in planning and interpreting experiments, as well as reading, presenting, and discussing literature and scientific articles. |
| Literatur | Lectures, protocols, other information, and the agenda will be provided using an internal platform and on ADAM (https://adam.unibas.ch). |
| Bemerkungen | Pregnant and nursing mothers should inform the assessor of the course at least two weeks prior to EMB course start. Students must have an accident insurance. Students requiring adaptive measures due to a handicap or chronic disorder should inform the assessor after registration. The “StoB (Studieren ohne Barrieren)” service center is available for your needs and assistance. In case of sickness, inform the assessor by mail with a letter of absence including a sick note (attest). Students are divided into two groups (group assignment will be communicated beginning of September): Group A: Tuesday-Wednesday, 13:15-18:00 Group B: Thursday-Friday, 13:15-18:00 All students of Group A and B will meet on September 17, 2024 at 13:15 for the introduction in room 02.073 (Biozentrum). For introduction into data management, we will host Dr. Michael Podvinec. For the introduction into microscopy, Dr. Oliver Biehlmaier and the Imaging Core Facility (IMCF) will run parts of the course as follows: • Introduction to microscopy and theory (teaching week for Group A and B): November 26 (Tuesday) and November 28 (Thursday) • Practical weeks from December 3-6 and December 10-13 (lab weeks for Group A and B as announced) |
| Teilnahmevoraussetzungen | Three requirements must be met for admission to the EMB course: 1. The practical course «Allgemeine Chemie» (12222) and «Grundlagen der präparativen organischen Chemie» (10571) must have been attended. The terms of participation and further information for these courses can be found online in the lecture directories. 2. You must have written the exams at least once in three out of six main lectures of module «Biologie 1» and «Biologie 2». 3. You must register for the EMB course in unibas online services no later than August 20, 2024 (https://services.unibas.ch/). REGISTRATION IS CLOSED. |
| Anmeldung zur Lehrveranstaltung | You must register for the EMB course in unibas online services no later than August 20, 2024 (https://services.unibas.ch/). REGISTRATION IS CLOSED. |
| Unterrichtssprache | Englisch |
| Einsatz digitaler Medien | kein spezifischer Einsatz |
| Intervall | Wochentag | Zeit | Raum |
|---|---|---|---|
| wöchentlich | Dienstag | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| wöchentlich | Mittwoch | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| wöchentlich | Donnerstag | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| wöchentlich | Freitag | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Bemerkungen |
Two afternoons per week (Tuesday and Wednesday or Thursday and Friday). Rooms and additional information for the first week will be announced by mail prior to EMB course start. |
Einzeltermine
| Datum | Zeit | Raum |
|---|---|---|
| Mittwoch 18.09.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Donnerstag 19.09.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Freitag 20.09.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Dienstag 24.09.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Mittwoch 25.09.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Donnerstag 26.09.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Freitag 27.09.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Dienstag 01.10.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Mittwoch 02.10.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Donnerstag 03.10.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Freitag 04.10.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Dienstag 08.10.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Mittwoch 09.10.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Donnerstag 10.10.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Freitag 11.10.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Dienstag 15.10.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Mittwoch 16.10.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Donnerstag 17.10.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Freitag 18.10.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Dienstag 22.10.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Mittwoch 23.10.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Donnerstag 24.10.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Freitag 25.10.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Dienstag 29.10.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Mittwoch 30.10.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Donnerstag 31.10.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Freitag 01.11.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Dienstag 05.11.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Mittwoch 06.11.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Donnerstag 07.11.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Freitag 08.11.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Dienstag 12.11.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Mittwoch 13.11.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Donnerstag 14.11.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Freitag 15.11.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Dienstag 19.11.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Mittwoch 20.11.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Donnerstag 21.11.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Freitag 22.11.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Dienstag 26.11.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Mittwoch 27.11.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Donnerstag 28.11.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Freitag 29.11.2024 | 13.15-18.00 Uhr | Dies Academicus |
| Freitag 29.11.2024 | 13.15-18.00 Uhr | Dies Academicus |
| Freitag 29.11.2024 | 13.15-18.00 Uhr | Dies Academicus |
| Dienstag 03.12.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Mittwoch 04.12.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Donnerstag 05.12.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Freitag 06.12.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Dienstag 10.12.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Mittwoch 11.12.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Donnerstag 12.12.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Freitag 13.12.2024 | 13.15-18.00 Uhr | Biozentrum, Computerraum Mango 02.081 |
| Freitag 13.12.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Montag 16.12.2024 | 13.15-18.00 Uhr | Biozentrum, Computerraum Mango 02.081 |
| Dienstag 17.12.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Mittwoch 18.12.2024 | 13.15-18.00 Uhr | Biozentrum, Computerraum Mango 02.081 |
| Mittwoch 18.12.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Donnerstag 19.12.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Freitag 20.12.2024 | 13.15-14.00 Uhr | Biozentrum, Hörsaal U1.101 |
| Freitag 20.12.2024 | 13.15-18.00 Uhr | Biozentrum, Praktikumslabor klein 02.029 |
| Module |
Modul: Computational Biology (Bachelorstudium: Computational Sciences) Modul: Molekularbiologie (Bachelorstudium: Biologie) |
| Prüfung | Lehrveranst.-begleitend |
| Hinweise zur Prüfung | To pass «Experimental Molecular Biology», the following points are required: • Attendance of class • Writing and documenting experiments in a lab journal on a weekly basis • Active participation in the lab and in class • Preparation and presentation of literature and research seminars • Leading good laboratory practice • 45-min exam at the end of the semester (20.12.2024, 13:15-14:00, U1.101) |
| An-/Abmeldung zur Prüfung | Anm.: Belegen Lehrveranstaltung; Abm.: stornieren |
| Wiederholungsprüfung | keine Wiederholungsprüfung |
| Skala | Pass / Fail |
| Belegen bei Nichtbestehen | beliebig wiederholbar |
| Zuständige Fakultät | Philosophisch-Naturwissenschaftliche Fakultät, studiendekanat-philnat@unibas.ch |
| Anbietende Organisationseinheit | Departement Biozentrum |