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12273-01 - Block course: Microbiology and Immunology (15 CP)
| Semester | fall semester 2025 |
| Course frequency | Every fall sem. |
| Lecturers |
Marek Basler (marek.basler@unibas.ch)
Dirk Bumann (dirk.bumann@unibas.ch, Assessor) Christoph Dehio (christoph.dehio@unibas.ch) Médéric Diard (mederic.diard@unibas.ch) Knut Drescher (knut.drescher@unibas.ch) Urs Jenal (urs.jenal@unibas.ch) Carolyn King (carolyn.king@unibas.ch) Jean Pieters (jean.pieters@unibas.ch) Daniel Pinschewer (daniel.pinschewer@unibas.ch) |
| Content | Biological topics in microbiology, molecular genetics and infection biology (5 weeks): Basic molecular and cellular concepts of (i) bacterial cell cycle control, (ii) bacterial differentiation, (iii) signal transduction and gene regulation in bacteria, (iv) bacterial heterogeneity, (v) bacterial pathogenesis and subversion of host cellular functions, with emphasis on type-IV and type-VI secretion systems, (vi) innate and adaptive immune responses to bacterial infection, (vii) industrial microbiology, (viii) evolutionary microbiology. Technical topics in microbiology, molecular genetics and infection biology: Sterile technique, light microscopy, bacterial and bacteriophage growth and titration, specific enrichment of bacteria from the environment, genetic manipulations of bacteria (transposon mutagenesis, general recombination, generalized transduction, mapping of mutations, antibiotic selection, conditional mutations), isolation of DNA, agarose-gel electrophoresis, PCR amplification of a gene, cloning of the gene and expression of the product, protein purification and analysis (SDS PAGE), cell culture and infection of cell lines, monitoring of bacterial invasion, monitoring of apoptosis, resistance to the bactericidal activity of complement, construction of bacterial knock-out mutants, bacterial conjugation, DNA microarray analyses, RNA isolation and quantitative RT-PCR analysis, green fluorescent protein and immunofluorescence staining and fluorescence microscopy, bacterial cell cycle synchronization techniques, flow cytometry, metabolic radiolabeling, immune-precipitation and immune-blot analysis, bacterial population dynamics in co-cultures. Biological topics in immunology (1 week): (i) The molecular and cellular concepts for the development of the innate and adaptive immune system. (ii) How does the immune system respond to foreign intruders by discriminating self/non-self. (iii) How does the immune system protect against viral infection. (iv) How do pathogens interact with and reprogram immune cells. Technical topics in immunology: Identification of major organs of the immune system of mice by immunohistochemistry. Fluorescence activated cell sorting (FACS) for detection of cell types and antigen-specific T lymphocytes. Quantification of antibodies by enzyme linked immune sorbent assay (ELISA) and neutralization assay. Analysis of pathogen-macrophage interaction; Detection of immune activation status by PCR. |
| Learning objectives | The course teaches basic laboratory practices and experimental techniques used in modern molecular microbiology, bacterial genetics, bacterial infection biology, and immunology. Accompanying lectures complement the biological and technical themes. |
| Comments | for biology students only compulsory attendance |
| Weblink | https://adam.unibas.ch |
| Admission requirements | Successful completion of the basic studies and the first year of the advanced level in the Bachelor of Biology. |
| Language of instruction | English |
| Use of digital media | No specific media used |
| Interval | Weekday | Time | Room |
|---|---|---|---|
| Block | See individual dates | ||
| Block | See individual dates | ||
| Comments |
Block course: 10.11.-19.12.2025, Monday to Friday, 8:00-18:00 A detailed schedule will be provided by the lecturers in due time. |
Dates
| Date | Time | Room |
|---|---|---|
| Monday 10.11.2025 | 08.00-18.00 | Biozentrum, Praktikumslabor gross 02.017 |
| Tuesday 11.11.2025 | 08.00-18.00 | Biozentrum, Praktikumslabor gross 02.017 |
| Wednesday 12.11.2025 | 08.00-18.00 | Biozentrum, Praktikumslabor gross 02.017 |
| Thursday 13.11.2025 | 08.00-18.00 | Biozentrum, Praktikumslabor gross 02.017 |
| Friday 14.11.2025 | 08.00-18.00 | Biozentrum, Praktikumslabor gross 02.017 |
| Monday 17.11.2025 | 08.00-18.00 | Biozentrum, Praktikumslabor gross 02.017 |
| Tuesday 18.11.2025 | 08.00-18.00 | Biozentrum, Praktikumslabor gross 02.017 |
| Wednesday 19.11.2025 | 08.00-18.00 | Biozentrum, Praktikumslabor gross 02.017 |
| Thursday 20.11.2025 | 08.00-18.00 | Biozentrum, Praktikumslabor gross 02.017 |
| Friday 21.11.2025 | 08.00-18.00 | Biozentrum, Praktikumslabor gross 02.017 |
| Saturday 22.11.2025 | 08.00-18.00 | ITS Wartung Netz |
| Saturday 22.11.2025 | 08.30-18.00 | ITS Wartung Netz |
| Saturday 22.11.2025 | 08.30-18.00 | ITS Wartung Netz |
| Sunday 23.11.2025 | 08.00-18.00 | ITS Wartung Netz |
| Sunday 23.11.2025 | 08.30-18.00 | ITS Wartung Netz |
| Sunday 23.11.2025 | 08.30-18.00 | ITS Wartung Netz |
| Monday 24.11.2025 | 08.00-18.00 | Biozentrum, Praktikumslabor gross 02.017 |
| Tuesday 25.11.2025 | 08.00-18.00 | Biozentrum, Praktikumslabor gross 02.017 |
| Wednesday 26.11.2025 | 08.00-18.00 | Biozentrum, Praktikumslabor gross 02.017 |
| Thursday 27.11.2025 | 08.00-18.00 | Biozentrum, Praktikumslabor gross 02.017 |
| Friday 28.11.2025 | 08.00-18.00 | Dies Academicus |
| Friday 28.11.2025 | 08.30-18.00 | Dies Academicus |
| Friday 28.11.2025 | 08.30-18.00 | Dies Academicus |
| Monday 01.12.2025 | 08.00-18.00 | Biozentrum, Praktikumslabor gross 02.017 |
| Tuesday 02.12.2025 | 08.00-18.00 | Biozentrum, Praktikumslabor gross 02.017 |
| Wednesday 03.12.2025 | 08.00-18.00 | Biozentrum, Praktikumslabor gross 02.017 |
| Thursday 04.12.2025 | 08.00-18.00 | Biozentrum, Praktikumslabor gross 02.017 |
| Friday 05.12.2025 | 08.00-18.00 | Biozentrum, Praktikumslabor gross 02.017 |
| Monday 08.12.2025 | 08.00-18.00 | Biozentrum, Praktikumslabor gross 02.017 |
| Tuesday 09.12.2025 | 08.00-18.00 | Biozentrum, Praktikumslabor gross 02.017 |
| Wednesday 10.12.2025 | 08.00-18.00 | Biozentrum, Praktikumslabor gross 02.017 |
| Thursday 11.12.2025 | 08.00-18.00 | Biozentrum, Praktikumslabor gross 02.017 |
| Friday 12.12.2025 | 08.00-18.00 | Biozentrum, Praktikumslabor gross 02.017 |
| Monday 15.12.2025 | 08.00-18.00 | Biozentrum, Praktikumslabor gross 02.017 |
| Tuesday 16.12.2025 | 08.00-18.00 | Biozentrum, Praktikumslabor gross 02.017 |
| Wednesday 17.12.2025 | 08.00-18.00 | Biozentrum, Praktikumslabor gross 02.017 |
| Thursday 18.12.2025 | 08.00-18.00 | Biozentrum, Praktikumslabor gross 02.017 |
| Friday 19.12.2025 | 08.00-18.00 | Biozentrum, Praktikumslabor gross 02.017 |
| Modules |
Majors: Molecular Biology and Integrative Biology (Bachelor's Studies: Biology (Start of studies before 01.08.2022)) Module: Block Course (Master's degree subject: Biology) Module: Specialization in Molecular Biology (Bachelor's Studies: Biology) |
| Assessment format | continuous assessment |
| Assessment details | written exam, 180 minutes 15.01.2026, 9-12 Uhr, Biozentrum, Hörsaal U1.131 |
| Assessment registration/deregistration | Reg.: course registration, dereg: cancel course registration |
| Repeat examination | no repeat examination |
| Scale | 1-6 0,5 |
| Repeated registration | as often as necessary |
| Responsible faculty | Faculty of Science, studiendekanat-philnat@unibas.ch |
| Offered by | Departement Biozentrum |