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34604-01 - Lecture with internship: Introduction to protein interaction analysis by affinity purification - mass spectrometry (1 CP)
| Semester | spring semester 2017 |
| Course frequency | Irregular |
| Lecturers |
Thomas Bock (thomas.bock@unibas.ch, Assessor)
Alexander Schmidt (alex.schmidt@unibas.ch) |
| Content | 3-day practical course on affinity purification - mass spectrometry (AP-MS) based investigation of protein-protein interactions and posttranslational modifications. This course focuses on the protein interaction analysis of protein phosphatase complexes, which have been linked to the regulation of a number of fundamental biological processes (e.g. cell growth, apoptosis and more) and are involved in cellular transformation during tumorgenesis. Day 1: Sample preparation Seminar: Introduction to AP-MS workflows, advantages/disadvantages of different protein affinity tags/enrichment strategies Hands-on training: Affinity purification of one target protein per student (each student can bring one additional protein of interest for purification via affinity resins), protein digestion over night. Day 2: Mass spectrometric analysis Seminar: Introduction to mass spectrometry (MS) and its applications in molecular biology Hands-on training: Solid-phase extraction of peptides, mass spectrometric measurement. Day 3: Data analysis Seminar: Introduction to MS-data analysis, principles of protein identification. |
| Comments | |
| Weblink | http://www.biozentrum.unibas.ch/educatio |
| Admission requirements | The courses are limited to a maximum of 6 participants. Der Kurs muss vollständig besucht werden, ansonsten können die CPs nicht vergeben werden. Achtung: Der Kurs vom 14. -16.03.2017 ist ausgebucht! (12.01.2017) |
| Course application | |
| Language of instruction | English |
| Use of digital media | No specific media used |
| Interval | Weekday | Time | Room |
|---|
No dates available. Please contact the lecturer.
| Modules |
Cycle I: Soft Skills (Master Molecular Biology) Doctorate Biochemistry: Recommendations (PhD subject: Biochemistry) Doctorate Biophysics: Recommendations (PhD subject: Biophysics) Doctorate Cell Biology: Recommendations (PhD subject: Cell Biology) Doctorate Computational Biology: Recommendations (PhD subject: Computational Biology) Doctorate Genetics: Recommendations (PhD subject: Genetics) Doctorate Microbiology: Recommendations (PhD subject: Microbiology) Doctorate Molecular Biology: Recommendations (PhD subject: Molecular Biology) Doctorate Neurobiology: Recommendations (PhD subject: Neurobiology) Doctorate Pharmacology: Recommendations (PhD subject: Pharmacology) Doctorate Structural Biology: Recommendations (PhD subject: Structural Biology) |
| Assessment format | continuous assessment |
| Assessment registration/deregistration | Reg.: course registration, dereg: cancel course registration |
| Repeat examination | no repeat examination |
| Scale | Pass / Fail |
| Repeated registration | as often as necessary |
| Responsible faculty | Faculty of Science, studiendekanat-philnat@unibas.ch |
| Offered by | Departement Biozentrum |