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21525-01 - Lecture: Introduction to NMR spectroscopy of proteins and other biomolecules (3 CP)
| Semester | fall semester 2024 |
| Course frequency | Every fall sem. |
| Lecturers | Daniel Häussinger (daniel.haeussinger@unibas.ch, Assessor) |
| Content | Instrumentation; labeling techniques and sample preparation; water suppression techniques; standard 2D and 3D(4D) pulse sequences for backbone and sidechain assignment; structure determination by NMR; relaxation and dynamics; residual dipolar couplings; paramagnetic relaxation enhancement; pseudo-contact shifts; protein-ligand and protein-protein interactions; techniques for large proteins. |
| Learning objectives | Comprehension of the basic concepts of protein NMR. Participants should be enabled to follow the literature in the field and critically assess the use of NMR in their own research. |
| Bibliography | J. Cavanagh et al., Protein NMR Spectroscopy, Wiley, 2007 P.J. Hore et al., NMR: The Toolkit, Oxford Chemistry Primer #92, Oxford University Press, 2000 S. M. Pascal, NMR Primer: An hsqc-based approach, IM Publications, 2008 S. Berger, S. Braun, 200 and more NMR experiments, Wiley, 2004 |
| Comments | Exercises will be provided to deepen the understanding on a practical level. |
| Admission requirements | Basic knowledge of small molecule NMR spectroscopy is very helpful, but not obligatory. |
| Course application | Register on services.unibas.ch |
| Language of instruction | English |
| Use of digital media | No specific media used |
| Interval | Weekday | Time | Room |
|---|---|---|---|
| wöchentlich | Wednesday | 13.15-16.00 | Chemie, Organische, Kleiner Hörsaal OC |
Dates
| Modules |
Doctorate Chemistry: Recommendations (PhD subject: Chemistry) Module Specialisation: Chemistry (Master's Studies: Nanosciences) Module: Organic Chemistry (Master's Studies: Chemistry) |
| Assessment format | continuous assessment |
| Assessment details | A short paper from recent primary literature will be provided and the students will have to answer a number of questions on the text. Reading time: 20 minutes, questionnaire: 60 minutes. |
| Assessment registration/deregistration | Reg.: course registration, dereg: cancel course registration |
| Repeat examination | no repeat examination |
| Scale | 1-6 0,5 |
| Repeated registration | as often as necessary |
| Responsible faculty | Faculty of Science, studiendekanat-philnat@unibas.ch |
| Offered by | Departement Chemie |