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73175-01 - Internship: Experimental Molecular Biology (6 CP)
| Semester | fall semester 2024 |
| Course frequency | Every fall sem. |
| Lecturers |
Oliver Biehlmaier (oliver.biehlmaier@unibas.ch)
Dominik Buser (dominik-pascal.buser@unibas.ch, Assessor) Alexia Loynton-Ferrand (alexia.ferrand@unibas.ch) Alexander Schier (alex.schier@unibas.ch) |
| Content | • Media and buffer preparation for Molecular Biology • Molecular cloning: polymerase chain reaction (PCR), endonuclease/restriction enzyme digestion, agarose gel electrophoresis, ligation, bacterial transformation, DNA plasmid isolation/purification, Sanger sequencing • Mammalian cell culture: Culturing, splitting, counting and seeding cells, plasmid transfection, protein expression, cell lysis • Protein analysis: Protein concentration determination, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), Western Blotting, Enhanced chemiluminescence (ECL) • Light and Fluorescence Microscopy: Basics skills with light microscope, immunofluoresence (IF) staining • Scientific data illustration • Image analysis • Preparation and presentation of collected data • Reading and understanding literature • Experimental design • Troubleshooting |
| Learning objectives | Aim of the Experimental Molecular Biology (EMB) course is to train Biology students for the gradual transition from a student to a scientist, including training technical as well as scientific/intellectual skills. By combining these skills, students will dissect the function, localization, and interaction network of a protein of interest. Technical skills include essential lab skills, starting with pipetting, making buffers, cloning, CRISPR/Cas9, protein expression in mammalian cell culture, standard biochemical techniques, as well as more advanced techniques such as microscopy and proteomics. Scientific/intellectual skills comprise sharpening student’s mindset in planning and interpreting experiments, as well as reading, presenting, and discussing literature and scientific articles. |
| Bibliography | Lectures, protocols, other information, and the agenda will be provided using an internal platform and on ADAM (https://adam.unibas.ch). |
| Comments | Pregnant and nursing mothers should inform the assessor of the course at least two weeks prior to EMB course start. Students must have an accident insurance. Students requiring adaptive measures due to a handicap or chronic disorder should inform the assessor after registration. The “StoB (Studieren ohne Barrieren)” service center is available for your needs and assistance. In case of sickness, inform the assessor by mail with a letter of absence including a sick note (attest). Students are divided into two groups (group assignment will be communicated beginning of September): Group A: Tuesday-Wednesday, 13:15-18:00 Group B: Thursday-Friday, 13:15-18:00 All students of Group A and B will meet on September 17, 2024 at 13:15 for the introduction in room 02.073 (Biozentrum). For introduction into data management, we will host Dr. Michael Podvinec. For the introduction into microscopy, Dr. Oliver Biehlmaier and the Imaging Core Facility (IMCF) will run parts of the course as follows: • Introduction to microscopy and theory (teaching week for Group A and B): November 26 (Tuesday) and November 28 (Thursday) • Practical weeks from December 3-6 and December 10-13 (lab weeks for Group A and B as announced) |
| Admission requirements | Three requirements must be met for admission to the EMB course: 1. The practical course «Allgemeine Chemie» (12222) and «Grundlagen der präparativen organischen Chemie» (10571) must have been attended. The terms of participation and further information for these courses can be found online in the lecture directories. 2. You must have written the exams at least once in three out of six main lectures of module «Biologie 1» and «Biologie 2». 3. You must register for the EMB course in unibas online services no later than August 20, 2024 (https://services.unibas.ch/). REGISTRATION IS CLOSED. For students in Nanosciences, please consult your students' office for course eligibility. |
| Course application | As Biology students, you must register for the EMB course in unibas online services no later than August 20, 2024 (https://services.unibas.ch/). REGISTRATION IS CLOSED. For students in Nanosciences, please consult your students' office for course eligibility. |
| Language of instruction | English |
| Use of digital media | No specific media used |
| Interval | Weekday | Time | Room |
|---|---|---|---|
| wöchentlich | Tuesday | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| wöchentlich | Wednesday | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| wöchentlich | Thursday | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| wöchentlich | Friday | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Comments | Two afternoons per week (Tuesday and Wednesday or Thursday and Friday, 13:15-18:00). Rooms and additional information for the first week will be announced by mail prior to EMB course start. |
Dates
| Date | Time | Room |
|---|---|---|
| Tuesday 17.09.2024 | 13.15-18.00 | Biozentrum, 02.073 |
| Wednesday 18.09.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Thursday 19.09.2024 | 13.15-18.00 | Biozentrum, 02.073 |
| Thursday 19.09.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Friday 20.09.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Tuesday 24.09.2024 | 13.15-18.00 | Biozentrum, 02.073 |
| Tuesday 24.09.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Wednesday 25.09.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Thursday 26.09.2024 | 13.15-18.00 | Biozentrum, 02.073 |
| Thursday 26.09.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Friday 27.09.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Tuesday 01.10.2024 | 13.15-18.00 | Biozentrum, 02.073 |
| Tuesday 01.10.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Wednesday 02.10.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Thursday 03.10.2024 | 13.15-18.00 | Biozentrum, 02.073 |
| Thursday 03.10.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Friday 04.10.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Tuesday 08.10.2024 | 13.15-18.00 | Biozentrum, 02.073 |
| Tuesday 08.10.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Wednesday 09.10.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Thursday 10.10.2024 | 13.15-18.00 | Biozentrum, 02.073 |
| Thursday 10.10.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Friday 11.10.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Tuesday 15.10.2024 | 13.15-18.00 | Biozentrum, 02.073 |
| Tuesday 15.10.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Wednesday 16.10.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Thursday 17.10.2024 | 13.15-18.00 | Biozentrum, 02.073 |
| Thursday 17.10.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Friday 18.10.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Tuesday 22.10.2024 | 13.15-18.00 | Biozentrum, 02.073 |
| Tuesday 22.10.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Wednesday 23.10.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Thursday 24.10.2024 | 13.15-18.00 | Biozentrum, 02.073 |
| Thursday 24.10.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Friday 25.10.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Tuesday 29.10.2024 | 13.15-18.00 | Biozentrum, 02.073 |
| Tuesday 29.10.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Wednesday 30.10.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Thursday 31.10.2024 | 13.15-18.00 | Biozentrum, 02.073 |
| Thursday 31.10.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Friday 01.11.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Tuesday 05.11.2024 | 13.15-18.00 | Biozentrum, 02.073 |
| Tuesday 05.11.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Wednesday 06.11.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Thursday 07.11.2024 | 13.15-18.00 | Biozentrum, 02.073 |
| Thursday 07.11.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Friday 08.11.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Tuesday 12.11.2024 | 13.15-18.00 | Biozentrum, 02.073 |
| Tuesday 12.11.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Wednesday 13.11.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Thursday 14.11.2024 | 13.15-18.00 | Biozentrum, 02.073 |
| Thursday 14.11.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Friday 15.11.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Tuesday 19.11.2024 | 13.15-18.00 | Biozentrum, 02.073 |
| Tuesday 19.11.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Wednesday 20.11.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Thursday 21.11.2024 | 13.15-18.00 | Biozentrum, 02.073 |
| Thursday 21.11.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Friday 22.11.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Tuesday 26.11.2024 | 13.15-18.00 | Biozentrum, 02.073 |
| Tuesday 26.11.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Wednesday 27.11.2024 | 13.15-18.00 | Biozentrum, 02.073 |
| Wednesday 27.11.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Thursday 28.11.2024 | 13.15-18.00 | Biozentrum, 02.073 |
| Thursday 28.11.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Friday 29.11.2024 | 13.15-18.00 | Dies Academicus |
| Tuesday 03.12.2024 | 13.15-18.00 | Biozentrum, 02.073 |
| Tuesday 03.12.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Wednesday 04.12.2024 | 13.15-18.00 | Biozentrum, 02.073 |
| Wednesday 04.12.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Thursday 05.12.2024 | 13.15-18.00 | Biozentrum, 02.073 |
| Thursday 05.12.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Friday 06.12.2024 | 13.15-18.00 | Biozentrum, 02.073 |
| Friday 06.12.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Tuesday 10.12.2024 | 13.15-18.00 | Biozentrum, 02.073 |
| Tuesday 10.12.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Wednesday 11.12.2024 | 13.15-18.00 | Biozentrum, 02.073 |
| Wednesday 11.12.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Thursday 12.12.2024 | 13.15-18.00 | Biozentrum, 02.073 |
| Thursday 12.12.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Friday 13.12.2024 | 13.15-18.00 | Biozentrum, 02.073 |
| Friday 13.12.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Tuesday 17.12.2024 | 13.15-18.00 | Biozentrum, 02.073 |
| Tuesday 17.12.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Wednesday 18.12.2024 | 13.15-18.00 | Biozentrum, 02.073 |
| Wednesday 18.12.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Thursday 19.12.2024 | 13.15-18.00 | Biozentrum, 02.073 |
| Thursday 19.12.2024 | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Friday 20.12.2024 | 13.15-14.00 | Biozentrum, U1.101 |
| Modules |
Electives Bachelor Nanosciences: Recommendations (Bachelor's Studies: Nanosciences) |
| Assessment format | continuous assessment |
| Assessment details | To pass «Experimental Molecular Biology», the following points are required: • Attendance of class • Writing and documenting experiments in a lab journal on a weekly basis • Active participation in the lab and in class • Preparation and presentation of literature and research seminars • Leading good laboratory practice • 45-min exam at the end of the semester (20.12.2024, 13:15-14:00, U1.101). |
| Assessment registration/deregistration | Reg.: course registration, dereg: cancel course registration |
| Repeat examination | no repeat examination |
| Scale | Pass / Fail |
| Repeated registration | as often as necessary |
| Responsible faculty | Faculty of Science, studiendekanat-philnat@unibas.ch |
| Offered by | Departement Biozentrum |