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34604-01 - Lecture with internship: Introduction to Protein Interaction Analysis by Affinity Purification - Mass Spectrometry (1 CP)
| Semester | fall semester 2025 |
| Course frequency | Every semester |
| Lecturers |
Thomas Bock (thomas.bock@unibas.ch, Assessor)
Alexander Schmidt (alex.schmidt@unibas.ch) |
| Content | 3-day practical course on affinity purification-mass spectrometry (AP-MS). During the course we will look into the methodology used for identification of protein-protein interactions by mass spectrometry. Specifically, we will perform the purification of a target protein and its preparation for mass spectrometry, we will perform the mass spectrometry measurement of the sample, as well as learn how to analyze the resulting AP-MS data. Day 1: Sample preparation Seminar: Introduction to AP-MS workflows, advantages/disadvantages of different protein affinity tags/enrichment strategies Hands-on training: Affinity purification of a target protein out of a cellular lysate, protein digestion over night Day 2: Mass spectrometry analysis Seminar: Introduction to mass spectrometry (MS) and its applications in molecular biology Hands-on training: Solid-phase extraction of peptides, mass spectrometry measurement Day 3: Data analysis Seminar: Introduction to MS-data analysis, principles of protein identification, protein-protein interaction data analysis |
| Comments | Please register via: phd-biozentrum@unibas.ch. Course dates: 04.11. - 06.11. 2025 |
| Weblink | Graduate Teaching Program |
| Admission requirements | The courses are limited to a maximum of 6 participants. PhD students only. The course must be fully attended, otherwise the CPs can not be awarded. |
| Language of instruction | English |
| Use of digital media | No specific media used |
| Interval | Weekday | Time | Room |
|---|---|---|---|
| Block | See individual dates | ||
Dates
| Date | Time | Room |
|---|---|---|
| Tuesday 04.11.2025 | 08.00-18.00 | --, -- |
| Wednesday 05.11.2025 | 08.00-18.00 | --, -- |
| Thursday 06.11.2025 | 08.00-18.00 | --, -- |
| Modules |
Cycle I: Practical and Experimental Skills (Master's Studies: Molecular Biology (Start of studies before 01.08.2025)) Doctorate Biochemistry: Recommendations (PhD subject: Biochemistry) Doctorate Biophysics: Recommendations (PhD subject: Biophysics) Doctorate Cell Biology: Recommendations (PhD subject: Cell Biology) Doctorate Computational Biology: Recommendations (PhD subject: Computational Biology) Doctorate Genetics: Recommendations (PhD subject: Genetics) Doctorate Microbiology: Recommendations (PhD subject: Microbiology) Doctorate Molecular Biology: Recommendations (PhD subject: Molecular Biology) Doctorate Neurobiology: Recommendations (PhD subject: Neurobiology) Doctorate Pharmacology: Recommendations (PhD subject: Pharmacology) Doctorate Structural Biology: Recommendations (PhD subject: Structural Biology) Module: Cycle I: Practical and Experimental Skills (Master's Studies: Molecular Biology) |
| Assessment format | continuous assessment |
| Assessment registration/deregistration | Reg.: course registration, dereg: cancel course registration |
| Repeat examination | no repeat examination |
| Scale | Pass / Fail |
| Repeated registration | as often as necessary |
| Responsible faculty | Faculty of Science, studiendekanat-philnat@unibas.ch |
| Offered by | Departement Biozentrum |