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| Semester | spring semester 2010 |
| Course frequency | Every spring sem. |
| Lecturers |
Cécile Arrieumerlou (cecile.arrieumerlou@unibas.ch)
Dirk Bumann (dirk.bumann@unibas.ch) Guy R. Cornelis (guy.cornelis@unibas.ch) Christoph Dehio (christoph.dehio@unibas.ch, Assessor) Daniela Finke (daniela.finke@unibas.ch) Urs Jenal (urs.jenal@unibas.ch) Malcolm G.P. Page (malcolm.page@basilea.com) Antonius G. Rolink (antonius.rolink@unibas.ch) |
| Content | Blockkurs Mikrobiobiologie und Immunologie Sommersemester, 3. Studienjahr, 15 Kreditpunkte Für das Bachelorstudium Biologie an der Universität Basel, Vertiefungsrichtung (Major) in Molecular Biology bzw. Integrative Biology Goals: The course is designed to teach the basic laboratory practices and experimental techniques used in modern molecular microbiology, bacterial genetics, bacterial infection biology and immunology. Accompanying lectures are designed to complement the following biological and technical themes. Biological themes for Microbiology, Molecular Genetics and Infection Biology (5 weeks) Basic molecular and cellular concepts of (i) bacterial cell cycle control, (ii) bacterial differentiation, (iii) signal transduction and gene regulation in bacteria, (iv) bacterial pathogenesis and subversion of host cellular functions, with emphasis on type-III and type-IV secretion systems, (v) innate immune responses to bacterial infection, and (vi) industrial microbiology. Technical themes for Microbiology, Molecular Genetics and Infection Biology Sterile technique, light microscopy, bacterial and bacteriophage growth and titration, specific enrichment of bacteria from the environment, genetic manipulations of bacteria (transposon mutagenesis, general recombination, generalized transduction, mapping of mutations, antibiotic selection, conditional mutations), isolation of DNA, agarose gel electrophoresis, PCR amplification of a gene, cloning of the gene and expression of the product, protein purification and analysis (SDS PAGE), cell culture and infection of cell lines, monitoring of bacterial invasion, monitoring of apoptosis, resistance to the bactericidal activity of complement, construction of bacterial knock-out mutants, bacterial conjugation, DNA microarray analyses, RNA isolation and quantitative RT-PCR analysis, green fluorescent protein and immunofluorescence staining and fluorescence microscopy, bacterial cell cycle synchronization techniques, flow cytometry, metabolic radiolabeling, immunoprecipitation and immunoblot analysis. Biological themes for Immunology (1 week) 1) The molecular and cellular concepts for the development of the innate and adaptive immune system. 2) How does the immune system remain reactive to foreign invaders, but tolerant to the bodys own structures and what are the potential scenarios in which self/non-self discrimination is lost. Technical themes for Immunology Identification of major organs of the immune system of mice and preparation of single cell suspensions. Tissue culture of lymphocytes and cytotoxic T cell analysis. Preparative and analytic fluorescence activated cell sorting (FACS). Purification of monoclonal antibodies, immunoprecipitation and functional testing. Detection and quantification of antigens by enzyme linked immunosorbent assay (ELISA). Dozierende für Mikrobiologie und Molekulargenetik: C. Arrieumerlou, D. Bumann, G. Cornelis, C. Dehio, U. Jenal, M. Page Dozierende für Immunologie: A. Rolink, D. Finke, J. Andersson |
| Weblink | http://www.biozentrum.unibas.ch/dehio/pr |
| Language of instruction | English |
| Use of digital media | No specific media used |
| Interval | Weekday | Time | Room |
|---|
No dates available. Please contact the lecturer.
| Modules |
Majors Molecular Biology and Integrative Biology (Bachelor Biologie) Modul Blockkurs (Master's degree subject: Biologie) |
| Assessment format | continuous assessment |
| Assessment details | voraussichtliches Prüfungsdatum: 22.04.2010 |
| Assessment registration/deregistration | Reg.: course registration; dereg.: teaching staff |
| Repeat examination | no repeat examination |
| Scale | 1-6 0,5 |
| Repeated registration | as often as necessary |
| Responsible faculty | Faculty of Science, studiendekanat-philnat@unibas.ch |
| Offered by | Departement Biozentrum |