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| Semester | spring semester 2024 |
| Course frequency | Annual course |
| Lecturers |
Dominik Buser (dominik-pascal.buser@unibas.ch, Assessor)
Alexander Schier (alex.schier@unibas.ch) |
| Content | • Repetition of acquired skills in "Experimental Molecular Biology (68795-01)" • Bacterial protein expression: IPTG induction and IMAC • Molecular cloning: DNA plasmid isolation/purification and Sanger sequencing • Mammalian cell culture: Culturing; Splitting; Counting and Seeding cells; Plasmid transfection; Protein expression; Cell lysis • Protein analysis: Protein concentration determination; Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE); Western Blotting; Enhanced chemiluminescence (ECL); Coomassie staining; Silver staining • Protein pulldown analysis • AP-MS/Proteomics: Theory and practice of mass spectrometry and proteomics; Pulldown with streptavidin beads; Reduction and tryptic digestion; Peptide purification and desalting; Preparation of samples for MS loading • Preparation and presentation of collected data • Reading, presenting, and understanding scientific articles • Experimental design • Troubleshooting |
| Learning objectives | Aim of the "Experimental Molecular Biology (EMB)" course is to train Biology students for the gradual transition from a student to a scientist, including training technical as well as scientific/intellectual skills. By combining these skills, students will dissect the function, localization, and interaction network of a protein of interest. Technical skills include essential lab skills, starting with pipetting, making buffers, cloning, protein expression in mammalian cell culture, standard biochemical techniques, as well as more advanced techniques such as microscopy and proteomics. Scientific/intellectual skills comprise sharpening student’s mindset in planning and interpreting experiments, as well as reading, presenting, and discussing literature and scientific articles. |
| Bibliography | Lectures, protocols, other information, and the agenda will be provided using an internal platform and on ADAM (https://adam.unibas.ch). |
| Comments | Pregnant and nursing mothers should inform the assessor of the course at least two weeks prior to EMB course start. Students must have an accident insurance. Students requiring adaptive measures due to a handicap or chronic disorder should inform the assessor after registration. The “StoB (Studieren ohne Barrieren)” service center is available for your needs and assistance. In case of sickness, inform the assessor by mail with a letter of absence including a sick note (attest). For introduction into proteomics, we will host Dr. Alex Schmidt and the Proteomics Core Facility. In addition, for a literature search seminar, we will host PD Dr. Christian Appenzeller-Herzog and Dr. Nuria Plattner. |
| Admission requirements | This course can be only attended if the "Experimental Molecular Biology (68795-01)" was completed (during fall semesters). |
| Course application | EMB course (68795-02) can be only attended if the former course (68795-01) has been completed. |
| Language of instruction | English |
| Use of digital media | No specific media used |
| Interval | Weekday | Time | Room |
|---|---|---|---|
| wöchentlich | Tuesday | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| wöchentlich | Wednesday | 13.15-18.00 | Biozentrum, Praktikumslabor klein 02.029 |
| Comments | Two afternoons per week (Tuesday and Wednesday). Rooms and additional information will be announced on our internal course website. |
| Modules |
Modul: Computational Biology (Bachelor's Studies: Computational Sciences) Module: Molecular Biology (Bachelor's Studies: Biology) |
| Assessment format | continuous assessment |
| Assessment details | To pass "Experimental Molecular Biology (68795-02)", the following points are required: • Attendance of class • Writing and documenting experiments in a lab journal on a weekly basis • Active participation in the lab and in class • Preparation and presentation of literature (Journal Club) and research seminars • Leading good laboratory practice "Experimental Molecular Biology (68795-02)" can be only attended after completion of "Experimental Molecular Biology (68795-01)". |
| Assessment registration/deregistration | Reg.: course registration, dereg: cancel course registration |
| Repeat examination | no repeat examination |
| Scale | Pass / Fail |
| Repeated registration | as often as necessary |
| Responsible faculty | Faculty of Science, studiendekanat-philnat@unibas.ch |
| Offered by | Departement Biozentrum |