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12273-01 - Block course: Microbiology and Immunology 15 CP

Semester fall semester 2024
Course frequency Every fall sem.
Lecturers Marek Basler (marek.basler@unibas.ch)
Dirk Bumann (dirk.bumann@unibas.ch, Assessor)
Christoph Dehio (christoph.dehio@unibas.ch)
Médéric Diard (mederic.diard@unibas.ch)
Knut Drescher (knut.drescher@unibas.ch)
Urs Jenal (urs.jenal@unibas.ch)
Carolyn King (carolyn.king@unibas.ch)
Jean Pieters (jean.pieters@unibas.ch)
Daniel Pinschewer (daniel.pinschewer@unibas.ch)
Content Biological topics in microbiology, molecular genetics and infection biology (5 weeks):
Basic molecular and cellular concepts of
(i) bacterial cell cycle control,
(ii) bacterial differentiation,
(iii) signal transduction and gene regulation in bacteria,
(iv) bacterial heterogeneity,
(v) bacterial pathogenesis and subversion of host cellular functions, with emphasis on type-IV and type-VI secretion systems,
(vi) innate and adaptive immune responses to bacterial infection,
(vii) industrial microbiology,
(viii) evolutionary microbiology.

Technical topics in microbiology, molecular genetics and infection biology:
Sterile technique, light microscopy, bacterial and bacteriophage growth and titration, specific enrichment of bacteria from the environment, genetic manipulations of bacteria (transposon mutagenesis, general recombination, generalized transduction, mapping of mutations, antibiotic selection, conditional mutations), isolation of DNA, agarose-gel electrophoresis, PCR amplification of a gene, cloning of the gene and expression of the product, protein purification and analysis (SDS PAGE), cell culture and infection of cell lines, monitoring of bacterial invasion, monitoring of apoptosis, resistance to the bactericidal activity of complement, construction of bacterial knock-out mutants, bacterial conjugation, DNA microarray analyses, RNA isolation and quantitative RT-PCR analysis, green fluorescent protein and immunofluorescence staining and fluorescence microscopy, bacterial cell cycle synchronization techniques, flow cytometry, metabolic radiolabeling, immune-precipitation and immune-blot analysis, bacterial population dynamics in co-cultures.

Biological topics in immunology (1 week):
(i) The molecular and cellular concepts for the development of the innate and adaptive immune system.
(ii) How does the immune system respond to foreign intruders by discriminating self/non-self.
(iii) How does the immune system protect against viral infection.
(iv) How do pathogens interact with and reprogram immune cells.

Technical topics in immunology:
Identification of major organs of the immune system of mice by immunohistochemistry. Fluorescence activated cell sorting (FACS) for detection of cell types and antigen-specific T lymphocytes. Quantification of antibodies by enzyme linked immune sorbent assay (ELISA) and neutralization assay. Analysis of pathogen-macrophage interaction; Detection of immune activation status by PCR.
Learning objectives The course teaches basic laboratory practices and experimental techniques used in modern molecular microbiology, bacterial genetics, bacterial infection biology, and immunology. Accompanying lectures complement the biological and technical themes.
Comments for biology students only
compulsory attendance
Weblink https://adam.unibas.ch

 

Admission requirements Successful completion of the basic studies and the first year of the advanced level in the Bachelor of Biology.
Language of instruction English
Use of digital media No specific media used

 

Interval Weekday Time Room
Block See individual dates
Comments Block course: 11.11.-20.12.2024

Dates

Date Time Room
Monday 11.11.2024 08.30-18.00 Biozentrum, Praktikum Vorlesungsraum 02.001
Tuesday 12.11.2024 08.30-18.00 Biozentrum, Praktikum Vorlesungsraum 02.001
Wednesday 13.11.2024 08.30-18.00 Biozentrum, Praktikum Vorlesungsraum 02.001
Thursday 14.11.2024 08.30-18.00 Biozentrum, Praktikum Vorlesungsraum 02.001
Friday 15.11.2024 08.30-18.00 Biozentrum, Praktikum Vorlesungsraum 02.001
Monday 18.11.2024 08.30-18.00 Biozentrum, Praktikum Vorlesungsraum 02.001
Tuesday 19.11.2024 08.30-18.00 Biozentrum, Praktikum Vorlesungsraum 02.001
Wednesday 20.11.2024 08.30-18.00 Biozentrum, Praktikum Vorlesungsraum 02.001
Thursday 21.11.2024 08.30-18.00 Biozentrum, Praktikum Vorlesungsraum 02.001
Friday 22.11.2024 08.30-18.00 Biozentrum, Praktikum Vorlesungsraum 02.001
Monday 25.11.2024 08.30-18.00 Biozentrum, Praktikum Vorlesungsraum 02.001
Tuesday 26.11.2024 08.30-18.00 Biozentrum, Praktikum Vorlesungsraum 02.001
Wednesday 27.11.2024 08.30-18.00 Biozentrum, Praktikum Vorlesungsraum 02.001
Thursday 28.11.2024 08.30-18.00 Biozentrum, Praktikum Vorlesungsraum 02.001
Friday 29.11.2024 08.30-18.00 Dies Academicus
Friday 29.11.2024 08.30-18.00 Dies Academicus
Monday 02.12.2024 08.30-18.00 Biozentrum, Praktikum Vorlesungsraum 02.001
Tuesday 03.12.2024 08.30-18.00 Biozentrum, Praktikum Vorlesungsraum 02.001
Wednesday 04.12.2024 08.30-18.00 Biozentrum, Praktikum Vorlesungsraum 02.001
Thursday 05.12.2024 08.30-18.00 Biozentrum, Praktikum Vorlesungsraum 02.001
Friday 06.12.2024 08.30-18.00 Biozentrum, Praktikum Vorlesungsraum 02.001
Saturday 07.12.2024 08.30-18.00 Biozentrum, Praktikum Vorlesungsraum 02.001
Sunday 08.12.2024 08.30-18.00 Biozentrum, Praktikum Vorlesungsraum 02.001
Monday 09.12.2024 08.30-18.00 Biozentrum, Praktikum Vorlesungsraum 02.001
Tuesday 10.12.2024 08.30-18.00 Biozentrum, Praktikum Vorlesungsraum 02.001
Wednesday 11.12.2024 08.30-18.00 Biozentrum, Praktikum Vorlesungsraum 02.001
Thursday 12.12.2024 08.30-18.00 Biozentrum, Praktikum Vorlesungsraum 02.001
Friday 13.12.2024 08.30-18.00 Biozentrum, Praktikum Vorlesungsraum 02.001
Saturday 14.12.2024 08.30-18.00 Biozentrum, Praktikum Vorlesungsraum 02.001
Sunday 15.12.2024 08.30-18.00 Biozentrum, Praktikum Vorlesungsraum 02.001
Monday 16.12.2024 08.30-18.00 Biozentrum, Praktikum Vorlesungsraum 02.001
Tuesday 17.12.2024 08.30-18.00 Biozentrum, Praktikum Vorlesungsraum 02.001
Wednesday 18.12.2024 08.30-18.00 Biozentrum, Praktikum Vorlesungsraum 02.001
Thursday 19.12.2024 08.30-18.00 Biozentrum, Praktikum Vorlesungsraum 02.001
Friday 20.12.2024 08.30-18.00 Biozentrum, Praktikum Vorlesungsraum 02.001
Modules Majors: Molecular Biology and Integrative Biology (Bachelor's Studies: Biology (Start of studies before 01.08.2022))
Module: Block Course (Master's degree subject: Biology)
Module: Specialization in Molecular Biology (Bachelor's Studies: Biology)
Assessment format continuous assessment
Assessment details written exam, 180 minutes
16.01.2025, 9-12 Uhr, Biozentrum, Hörsaal U1.131
Assessment registration/deregistration Reg.: course registration, dereg: cancel course registration
Repeat examination no repeat examination
Scale 1-6 0,5
Repeated registration as often as necessary
Responsible faculty Faculty of Science, studiendekanat-philnat@unibas.ch
Offered by Departement Biozentrum

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