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38238-01 - Internship: Basics in recombinant protein production (3 CP)
| Semester | fall semester 2024 |
| Course frequency | Every fall sem. |
| Lecturers |
Daniel Häussinger (daniel.haeussinger@unibas.ch)
Florian Peter Seebeck (florian.seebeck@unibas.ch, Assessor) |
| Content | Bio-based technologies will play a central role in future industries. Microorganisms have diversified for 3.5 billion years and evolved molecular systems to utilize different energy forms, to build complex molecular structure from diverse feedstocks and to recycle any type of biomass to basic minerals. We can exploit this molecular diversity as a toolbox for sustainable and innovative bio-based production of energy, materials, fine chemicals, food, or pharmaceuticals. In this context, recombinant protein production is a key technology because it allows the researcher access virtually any protein encoded by over 10000 sequenced microbial genomes. This course should train interested beginners in recombinant protein production techniques. |
| Learning objectives | This course is a first introduction to production and characterization of recombinant proteins. This course should enable students to search specific protein sequences by structure based genome mining, visualize protein structures, produce the corresponding protein in the lab, and analyze its homogeneity, stability and activity by a series of basic techniques. |
| Bibliography | Clark & Pazdernik: Molekulare Biotechnologie, Spektrum Verlag Click, Pasternak & Patten: Molecular Biotechnology, ASM press Lesk: Bioinformatik, Spektrum Verlag Sezonov, Joseleau-Petit, Dari, J. Bacteriol. 2007, 189 (23) 8746 – 8749 Studier, Protein Expr Purif. 2005, 41(1) 207 – 34 Carlson et al.: J Biol Chem. 2008, 283 (29) 20117 – 20125 Pace, Vajdos, Fee, Grimsley & Gray: Protein Sci. 1995, 4 (11), 2411 – 2423 Riener, Kada & Gruber: Anal. Bioanal. Chem. 2002, 373, 266 – 76 |
| Comments | Der Kurs ist auf sechs Teilnehmer beschränkt. Der zehntägige Kurs findet vom 29. Januar bis 09. Februar 2024 im Rosental-Areal statt. Es handelt sich um ein Vollzeitpraktikum. |
| Admission requirements | Bachelor degree in Chemistry/Nanosciences and related areas. |
| Course application | Please indicate your interest by email (florian.seebeck@unibas.ch) this will help us to plan the lab course. Also you need to register on services.unibas.ch. |
| Language of instruction | English |
| Use of digital media | No specific media used |
| Interval | Weekday | Time | Room |
|---|
No dates available. Please contact the lecturer.
| Modules |
Module Specialisation: Chemistry (Master's Studies: Nanosciences) Module: Mixed Topics (Master's Studies: Chemistry) |
| Assessment format | continuous assessment |
| Assessment details | The students will present their results in a group seminar at the end of the course and are requested to submit a written report. |
| Assessment registration/deregistration | Reg.: course registration, dereg: cancel course registration |
| Repeat examination | no repeat examination |
| Scale | Pass / Fail |
| Repeated registration | as often as necessary |
| Responsible faculty | Faculty of Science, studiendekanat-philnat@unibas.ch |
| Offered by | Departement Chemie |